Presentation description

Our research explores Abscission Checkpoint Bodies (ACBs), organelles that regulate cell division by ensuring proper membrane separation, the final step of cytokinesis (Strohacker et al.). We use TurboID, a biotin ligase, to tag and identify proteins in ACBs in living cells, employing DLD1 Nup153AIDGFP-TurboID-HA and TetOne SRRM2NT-TurboID-HA cell lines. Our results demonstrate TurboID's effectiveness in labeling proteins in ACBs and highlight the need to adjust doxycycline exposure for better detection, advancing our understanding of protein regulation in cell division.