Presentation description

Looked at the role of NFAT5 in renal epithelial cells and macrophages. Using a Crispr NFAT5 knockout line of IMCD cells, I did experiments looking at the survival of IMCD NFAT5 knockout cells and Wt IMCD cells and qPCR looking at transporter expression in varying osmolarities and osmolites. Then performed qPCR of RAW cells, using si-rna to knock down NFAT5 expression.