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Enzymatic Formation of Multi-Crosslinked Peptide Architectures and Processing of Xenobiotic Substrates

Semester: Summer 2023


Presentation description

PapB is a highly promiscuous radical S-adenosyl-l-methionine (rSAM) enzyme that can install thio(seleno)ether crosslinks in diverse substrates. The substrate scope of this enzyme has been largely underexplored, with most studies only considering the native Cys-X3-Asp thioether motif. The redox stability of thioether crosslinks makes PapB an attractive candidate for use as a biotechnological tool. The results demonstrated here show that PapB will process nested and in-line crosslinks with varied distances between each crosslinking site. Further, we demonstrate that PapB will process non-peptidic substrates and install the thioether crosslink at predictable positions.

Presenter Name: Kian Barnes

Presentation Type: Poster
Presentation Format: In Person
Presentation #55
College: Science
School / Department: Chemistry
Research Mentor: Vahe Bandarian
Date | Time: Thursday, Aug 3rd | 10:30 AM