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Expressing and Purifying Type IV CRISPR Accessory Proteins

Year: 2023

Presenter Name: Alivia Jolley

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)-Cas (CRISPR-associated) adaptive immune systems defend bacteria and archaea against phages, plasmids, and other mobile genetic elements. Each Type IV CRISPR system contains a subtype-specific gene and is hypothesized to be essential for function. The Type IV-A system encodes ATP-dependent 5'-3' DNA helicase called CasDinG, while the Type IV-B systems encode a putative pyrophosphatase named CasCysH. Here we investigate the function of the type IV CRISPR systems by expressing, purifying, and characterizing the accessory proteins from the type IV systems. This project is a first step towards understanding the structure and mechanism of type IV systems and the possibility of repurposing type IV systems in future applications.
University / Institution: Utah State University
Type: Poster
Format: In Person
Presentation #C7
SESSION C (1:45-3:15PM)
Area of Research: Science & Technology
Faculty Mentor: Ryan Jackson