Presentation description

In this proposed research, our focus centers on unraveling the intricacies of the BMPER (Bone Morphogenetic Protein-binding Endothelial cell precursor-derived Regulator) gene within the context of adipose tissue homeostasis. BMPER is expressed prominently in APC's (Adipose tissue Progenitor Cells) in both humans and mice. The primary objective of this study is to validate the pro-adipogenic effects of BMPER, showing its contribution to the differentiation and maturation of adipocytes. Additionally, we aim to delve into the gene's role in metabolic regulation. Our approach involves an examination of BMPER expression patterns and interactions within adipose tissue progenitor cells. Furthermore, the study will incorporate ways to assess insulin sensitivity, providing a clear understanding of BMPER's involvement in metabolic homeostasis. By delving into the mechanisms about BMPER in adipose tissue, this research contributes to the fundamental understanding of adipogenesis, homeostasis, and insulin sensitivity.We suggest that the BMPER gene significantly influences adipose tissue and metabolic health. Our hypothesis suggests that BMPER, found prominently in Adipose Tissue Progenitor Cells (APCs), plays a crucial role in guiding the development and maturation of fat cells. We anticipate that by confirming the pro-adipogenic effects of BMPER, we will uncover its importance in maintaining metabolic balance. Through tests measuring insulin sensitivity, qPCR, BODIPY (staining that will help us determine whether BMPER is necessary and sufficient for 3T3-L1 cells to accumulate lipids as they differentiate into adipocytes), and Western Blot to assess BMPER protein expression during differentiation. If our hypothesis is correct, it could offer valuable insights into addressing metabolic issues related to obesity. By understanding BMPER's role, we may identify new strategies for preventing and treating conditions linked to cardiovascular risk in for obese individuals. This research contributes to a broader understanding of how fat cells (adipocytes) develop and maintain balance.