Presentation description

Here, we explored the QF transcription factor activation mechanism in prokaryotes by characterizing the behavior of a fusion protein (TetR-QFAD) consisting of the tetracycline repressor (TetR) and the QF activating domain (QFAD) downstream of the T7 promoter. We demonstrate that when placed downstream of the T7 promoter, TetR-QFAD functions as a repressor and an activator depending on the presence of aTc.