Presentation description

I used bioluminescence resonance energy transfer (BRET) to investigate interactions between different proteins in the Hedgehog (Hh) pathway. I focused on Kif7 and SuFu interactions with the GPCR Smoothened (SMO) and PKA-C. Kif7 is analogous to Cos2 in fruit flies. Cos2 scaffolds the GLI transcription factor and PKA-C. I suspect that Kif7 may play a similar role. Kif7 and SuFu may also play a role in PKA-C binding to SMO.