Presentation description

ZBTB4 is part of a methyl-CpG binding protein family involved in regulating normal and disease relevant transcription. While the N-terminal zinc fingers (ZFs) are known to provide methyl-specific DNA readout, the role of the C-terminal ZF domain was undefined. We discovered this domain recognizes G-quadruplex DNA. A combination of solution NMR and electrophoretic mobility shift assays have been used to characterize this binding; which expands understanding of ZBTB4 transcriptional activities.