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This project is about understanding the metabolic dependencies and localization of the Calreticulin (CALR) protein in a cell. CALR is a protein normally located in the endoplasmic reticulin. It functions as a chaperone protein. When this protein is mutated it is associated with myeloproliferative neoplasms or MPNS. Therefore, understanding CALR's metabolic dependencies will allow us to understand how to treat it. This is done by first isolating the protein then performing mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically or MIDAS. MIDAS is a process that causes a protein to interact with specific metabolites. Another way of understanding the metabolic decencies of CALR is to know if it localizes to the mitochondria. This is done with a mitochondria isolation kit.