Presentation description

The visualization of RNA in live cells is critical for understanding its dynamics, localization, and function. Fluorogen-aptamer systems have emerged as powerful tools for noninvasive RNA imaging; however, many existing systems are limited by poor photostability and low brightness. The recently developed Okra aptamer, in complex with the ACE fluorogenic dye, has demonstrated enhanced photophysical properties, including improved fluorescence emission and photostability, enabling its application in super-resolution microscopy. In this work, we report the synthesis of a π-extended derivative of the ACE dye and its interaction with the Okra aptamer. The modified aptamer-dye system exhibits a red-shifted emission, expanding the fluorescence spectrum. These results suggest the modified system offers a broader range of imaging capabilities for real-time, making it very versatile for different studies of RNA visualization.