Presentation description

The interaction between OCT1 (Octamer-binding transcription factor 1) and OCA-B (Octamer-binding transcription factor co-activator B) regulates gene expression linked to cell proliferation, differentiation, and survival, crucial in cancer development. Regulation of Oct1/OCA B interaction is a novel therapeutic target for controlling transcription hence related diseases. Assay development for studying Oct1/OCA B protein-protein interaction within cells would be extremely useful for validation of small molecule inhibitors/activators to find novel therapeutics targeting this transcriptional complex, contributing significantly to cancer biology and related diseases. In this study we have designed and developed the nanoBRET assay system using latest molecular biology technique to measure the inhibition or stabilization of Oct1/OCA B protein-protein interaction by small molecule within cells for drug discovery research.