Presentation description

SF3B1 is an essential RNA splicing protein that normally resides in the nucleus. In our study, we observed that when NUP153 a key part of the nuclear pore complex is knocked down, SF3B1 mislocalizes to the cytoplasm, especially during cytokinesis. Since SF3B1 has a KRKRR sequence similar to a nuclear localization signal (NLS), we tested whether faulty nuclear import was the cause by replacing it with a stronger SV40-type signal (KKKRK). However, this did not prevent mislocalization, suggesting other factors are involved. To explore this further, we created a toolkit of full-length SF3B1 constructs tagged at the N-terminus with either GFP or HA. These were assembled using Gibson Assembly and verified through sequencing.