Faculty mentor: Matthew Kieber-Emmons
Photoactive yellow protein (PYP), a light sensing protein found in Halorhodospira halophila, undergoes a unique structural change in response to blue light. PYP is believed to play a role in signaling the negative phototactic behavior of the bacteria; however, its signaling partner has not been identified. This work describes the adaptation of the Bacterial Adenylate Cyclase Two-Hybrid method to test the interactions of PYP with potential partner proteins and elucidate its signaling mechanism.
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