Faculty mentor: Dr. Randall T. Peterson
CRISPR-Cas9 has simplified gene knockouts. However, large-scale CRISPR screens in vivo haven’t been feasible yet. We’ve developed a platform for genome-scale functional genetic screens in zebrafish. It uses microfluidics to generate nanodroplets with Cas9, sgRNA & a unique barcode for each target gene (TG). The screen is done by microinjection of one droplet per embryo from a pooled droplet library. Embryos expressing a desired phenotype are lysed and the TG identified by recovering the barcode.
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