Poster 121: Calder Duffin – Probing Peptide Substrate Recognition of Radical S-Adenosyl Methionine Enzyme Tte1186 via Assay of Synthesized Peptide Substrate Mutants and Liquid Chromatography/Mass Spectroscopy Analysis

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Faculty mentor: Vahe Bandarian

Mutant peptide substrates for the radical SAM enzyme Tte1186 were synthesized and assayed for reactivity to probe the pattern of recognition for the maturase enzyme to be used as a notion for other radical SAM enzymes that create mature RiPPs. Assays were analyzed via LC/MS to verify cross-link formation.

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