Faculty mentor: Aylin Rodan
The iono- and osmoregulatory epithelia of Drosophila melanogaster, the Malpighian tubules and hindgut, contain ion transporters and channels that maintain homeostasis in the fly. Compared to the Malpighian tubule, ion transporters and channels in the hindgut have not been well characterized. The goal is to knock down expression of the ion transporters IRK2 and NCC69 in the hindgut using the GAL4/UAS system and RNA interference, and analyze the effects on Drosophila excretion and osmoregulation.
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