SPUR 2021: INDUCED PLURIPOTENT STEM CELL DERIVED CARDIOMYOCYTE MODELS OF HUMAN CARDIOVASCULAR DISORDERS

Faculty Name:
Martin Tristani-Firouzi

Department:
Pediatrics

Faculty College:
Medicine

Email:


Project Description:

**This project is a part of the Summer Program for Undergraduate Research (SPUR), which provides undergraduate students with an intensive 10-week research experience under the mentorship of a University of Utah faculty member. SPUR 2021 begins on May 26 and ends on August 5. If you are interested in this project, please review all program information on the SPUR site. If you wish to apply to this project, you must apply using the SPUR 2021 application.**

Advances in induced pluripotent stem cell (iPSC) technology allow for reprogramming of adult somatic cells into stem cells from which patient-specific (and thus disease-specific) cardiomyocytes (CM) can be derived. The impact of this technology has far-reaching implications, ranging from drug discovery, pre-clinical drug screening, mechanistic understanding of disease processes, and advances in personalized medicine. Specifically human iPSC-CMs offer a unique model system to study phenotypic variation in Long QT Syndrome (LQTS), by allowing for direct comparisons of gene expression in human cardiac cells derived from family members who share the same mutation, but manifest severe versus mild symptoms. However, the potential of iPSC technology to answer this and other crucial research questions is limited by several technological barriers. For example, human iPSC-CMs in culture behave more like embryonic than mature myocytes and thus may not recapitulate key features of childhood or adult-onset disease. The overall goal of this proposal is to overcome the maturational hurdles in order to design a valid iPSC-CM model system for the study human arrhythmia disorders. This proposal utilizes cutting-edge technological approaches that will advance the field of cardiovascular medicine in a very practical manner.

The stipend for this SPUR project is funded by an American Heart Association grant awarded to Dr. Stavros Drakos, MD, PhD. The stipend for this project is $4,000 instead of $5,000 due to grant funding limitations.



Opportunity Type:

This is a paid research position


Student Role:

The student will be responsible for differentiating stem cells into beating myocytes and will learn basic tissue culture techniques. The student may be involved in designing systems to improve the maturation of cardiomyoyctes in culture. In addition, the student will perform biochemical and molecular biology assays, including single cell RNA-seq experiments and analyses. The student will be paired with one or two post-doctoral fellows who will provide daily mentoring and support. Remote Contingency Plan: This project does not a have a remote contingency plan. While students from other institutions may apply for this project, preference may be given to University of Utah students and students local to the Salt Lake City area depending on travel and/or on-campus research restrictions at the time of award notifications.


Student Benefits:

The student will gain exceptional experiences in different areas of basic and translational biomedical research. She/he will have an opportunity to gain insights into arrhythmia disorders, biochemical and molecular regulation of cardiac ion channels. The student will have plenty of hands-on involvement in conducting laboratory research and learn a great deal about stem cell models of human disease. This research opportunity is well suited for student who would like to pursue a medical career and research in biomedical science (MD/PhD). The learning experience from this project will help and encourage the undergraduate student to prepare for a research related career and professional development to become a scientist and/or physician.


Project Duration:

35-40 hours per week on research and program-related activities, begins May 26, 2021, and ends August 5, 2021


Minimum Requirements:

Admission to the program is competitive. Applicants must meet all of the following criteria: 1) be a matriculated, degree-seeking undergraduate student in the Fall 2021 semester (beginning or continuing college career in Fall 2021 and not graduating before December 2021; concurrent enrollment while in high school does not meet this eligibility requirement). Applicants do not need to be a University of Utah student. 2) eligible to work in the United States: If you are a University of Utah Dreamer (with or without DACA), you are eligible to participate. If you are a Dreamer from a different institution: If you have DACA, you are eligible to participate. If you do not have DACA, you are able to participate and gain research experience, but might not be able to be compensated. For more information, please contact Megan Shannahan at megan.shannahan@utah.edu or 801-581-2478. If you are an international student or scholar, you must either a) be a degree-seeking undergraduate student at an American institution of higher education and verify with your institution’s international center that your visa allows you to participate in this program, OR b) possess documentation that establishes your eligibility to work in the United States (if you hold US citizenship, it is likely you have these documents). 3) able to commit to approximately 35-40 hours per week of employment at the University of Utah for the entire duration of the program (May 26-August 5, 2021). 4) at least 18 years old by May 24, 2021 (required if you wish to use on-campus housing; preferred if you will not be using on-campus housing). Please note that no previous college coursework or previous research experience is required.