SPUR 2021: EFFECTS OF ALTITUDE ON BRAIN EXCITABILITY AND MIGRAINE

Faculty Name:
KC Brennan

Department:
Neurology

Faculty College:
Medicine

Email:


Project Description:

**This project is a part of the Summer Program for Undergraduate Research (SPUR), which provides undergraduate students with an intensive 10-week research experience under the mentorship of a University of Utah faculty member. SPUR 2021 begins on May 26 and ends on August 5. If you are interested in this project, please review all program information on the SPUR site. If you wish to apply to this project, you must apply using the SPUR 2021 application.**

Altitude affects the brain as well as the lungs. It is well known that high altitudes can cause severe headaches and ultimately brain swelling. It is less known that moderate altitudes, like that of Salt Lake City (SLC), can also have effects on the brain. This is important because approximately 1 billion people live at moderate altitudes. Migraine headaches are more common at moderate altitudes, and we have recently shown that migraine aura, a wave of sensory changes that precedes the headache, is significantly more frequent in people who live in SLC compared to lower elevations. This is an important clue because we know that migraine aura is caused by a spreading depolarization (SD), a wave of activity that moves across the brain like a ripple in a pond. While the pain of migraine is difficult to model in the lab, SD is readily measured. We thus have a biomarker that we can use to examine the mechanisms by which altitude affects migraine susceptibility. We have two potentially related hypotheses: 1. Hypoxia, reduced oxygen concentration, is known to trigger SD. Though there are only moderate changes in oxygen levels in SLC and other moderate elevations, they are physiologically significant, and may be sufficient to trigger SD. 2. Red blood cell mass increases significantly, even at moderate elevations. Though this helps deliver oxygen, it also makes the blood thicker and more likely to clot. Even transient clotting can trigger SD. Using optical techniques, we are able to directly visualize blood oxygenation, blood flow, and clotting, in real time in awake animals. We will use these techniques, along with an altitude chamber, to test our hypotheses in wild type mice, and in mutants carrying migraine genes.



Opportunity Type:

This is a paid research position


Student Role:

The student will be able to participate in all aspects of the project, and we can tailor their experience based on their skill set and interests. For example, a student with an interest in biomedical engineering will have the chance to work on an altitude chamber, and with cutting edge optics, including spectroscopy and two photon imaging. A student with interest in sensory neurobiology will be in the position to image neurons in the brain of an awake behaving mouse. Ideally, we will be able to convince our student that both the engineering and neuroscience aspects of our work are fascinating. Remote Contingency Plan: About half of our work - the non-experimental aspect, can be performed from anywhere, with a laptop computer. This half includes data analysis, generation of figures, writeup, and presentation. There are also aspects to the experimental work that can actually be done remotely; for example we can remotely deliver supplies and devices, in order that a student be able to construct an experimental apparatus - in the case of this proposal, an altitude chamber. We want to have a balance of experimental and analytical work, because both are necessary, and neither alone is sufficient, for scientific training. Thus, if the project has to be virtual, we will make sure that at least a third of the work is hands-on, experimental work that can be done remotely. Beyond the altitude chamber work, we have a small 3D printer that can be cleaned and delivered to a student's home, so they can build experimental tools that others (and hopefully they after quarantine) can deploy in lab.


Student Benefits:

Whether headed toward a scientific career or medical school, the key thing to be able to show from lab experience is publications. This is because a paper is the culmination of many other research-related goals: understanding the scientific literature, knowing laboratory or clinical techniques, generating a dataset, analyzing the data, presenting it at conferences, and writing it up. It is a credible demonstration that the student did ‘real work’ and is being rewarded for it. So this is our primary, focusing, practical goal. But there is more to a lab experience than generating publication quality research. There is the critical skill of learning how to learn; there is no textbook when you are at a knowledge frontier, and this skill is a profound help in any field. Finally there is the confidence that comes from solving problems, and the joy of discovering something truly new.


Project Duration:

35-40 hours per week on research and program-related activities, begins May 26, 2021, and ends August 5, 2021


Minimum Requirements:

Admission to the program is competitive. Applicants must meet all of the following criteria: 1) be a matriculated, degree-seeking undergraduate student in the Fall 2021 semester (beginning or continuing college career in Fall 2021 and not graduating before December 2021; concurrent enrollment while in high school does not meet this eligibility requirement). Applicants do not need to be a University of Utah student. 2) eligible to work in the United States: If you are a University of Utah Dreamer (with or without DACA), you are eligible to participate. If you are a Dreamer from a different institution: If you have DACA, you are eligible to participate. If you do not have DACA, you are able to participate and gain research experience, but might not be able to be compensated. For more information, please contact Megan Shannahan at megan.shannahan@utah.edu or 801-581-2478. If you are an international student or scholar, you must either a) be a degree-seeking undergraduate student at an American institution of higher education and verify with your institution’s international center that your visa allows you to participate in this program, OR b) possess documentation that establishes your eligibility to work in the United States (if you hold US citizenship, it is likely you have these documents). 3) able to commit to approximately 35-40 hours per week of employment at the University of Utah for the entire duration of the program (May 26-August 5, 2021). 4) at least 18 years old by May 24, 2021 (required if you wish to use on-campus housing; preferred if you will not be using on-campus housing). Please note that no previous college coursework or previous research experience is required.