Determine the role of Abcb10 in red cell hemoglobinization using tissue culture, metabolic analyses and transcriptional profiling. Abcb10 is a mitochondrial membrane transporter that utilizes ATP hydrolysis to export a metabolite out of the mitochondria. This process is necessary for developing red cell hemoglobinization. We have generated a tissue culture cell line that has lost expression of Abcb10. These cells do not hemoglobinize. We will determine changes in mitochondrial metabolites and gene expression.
Diane McVey Ward
Volunteer; This is a paid research position; Prepare a UROP proposal
An undergraduate will work directly with a lab technician and the PI to culture cells, transduce cells with a lentivirus, isolate mitochondria and analyze metabolomic data
Students will learn the workings of a research lab, learn common research techniques, review literature and assist in figure and manuscript preparation.
The project will be for one - two years depending upon progress and the student will work up to 20 hrs/week.
Students must have courses in organic chemistry and preferrably cell biology with added benefit if they have taken biochemistry.