CRISPR edited megakaryocytes to screen platelet gene functions

Mentor Name:
Jesse Rowley

Mentor Position:
Research Assistant Professor

Internal Medicine



Project Description:

Platelets are small white blood cells that help prevent excessive bleeding. To stop bleeding, platelets activate, stick to damaged blood vessels and aggregate to form a clot. On the flip side, platelets also can promote dangerous clot formation such as in heart attacks and strokes. Several genes have been associated with clotting. We are using CRISPR/CAS9 gene editing in human megakaryocytes (the cells that make platelets) grown in a dish or in mice to test the role of these genes in platelet function.

Opportunity Type:

Volunteer; This is a work-study research position; Prepare a UROP proposal; Write an Honors Thesis or Senior Thesis

Student Role:

The student may help with blood cell isolation, human cell culture, perform CRISPR/CAS9 transfections, polymerase Chain Reaction (PCR), microscopy, and mouse experiments.

Student Benefits:

Student will learn to design experiments and perform laboratory procedures and techniques. Students will have the opportunity to present their results in papers and posters.

Project Duration:

8+ hours per week for > 1 year.

Minimum Requirements:

Must be able to work two or more 4 hour blocks per week, 2 consecutive days preferred. Some chemistry and math skills required (dilutions, calculating molarity, percentages).