Faculty mentor: Robby Bowles
Using CRISPR-dCas9-VPR we upregulated genes in adipose-derived stem cells to see if any played a role in osteogenesis. CRISPR-dCas9-VPR is a modified form of CRISPR-Cas9, instead of cutting DNA it attaches onto and helps the cell express the gene following the place of attachment. We used a modified cell line of adipose-derived stem cells that would fluoresce if undergoing bone cell differentiation. After upregulating thousands of genes we noticed some had an effect on osteogenesis.
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