Poster #6: BRYCE LARSEN – Promoting Osteogenesis using CRISPR-dCas9-VPR

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Faculty mentor: Robby Bowles

Using CRISPR-dCas9-VPR we upregulated genes in adipose-derived stem cells to see if any played a role in osteogenesis. CRISPR-dCas9-VPR is a modified form of CRISPR-Cas9, instead of cutting DNA it attaches onto and helps the cell express the gene following the place of attachment. We used a modified cell line of adipose-derived stem cells that would fluoresce if undergoing bone cell differentiation. After upregulating thousands of genes we noticed some had an effect on osteogenesis.

Click below to hear me present my poster!
Questions or comments? Contact me at: bryce.verl.larsen@gmail.com

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