Oral #16: Sophie Stephens – Identifying primary cells adherent to the arterial sheath of patients undergoing left-heart catheterization

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Faculty mentor: Dave Symons

The purpose of my project is to develop a procedure to obtain pure primary endothelial cells (ECs) from patients with cardiovascular disease. The sheath that is otherwise discarded after a left-heart catheterization procedure is obtained, and the adherent cells are collected and incubated with fluorescent-conjugated antibodies specific to ECs, leukocytes and other various cell types. The cell admixture is stained with dapi and the samples are sorted via fluorescence-activated cell sorting.

Watch my research presentation below.
Questions or comments? Contact me at: slslou35@gmail.com

View my Presentation Slides HERE 

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